Seitenhistorie

Service contact (administrators only): "EVO Portal", Perkin Elmer website.

Skirt height
Difference between plate-edge height, and imaging surface height
Important value to enter into machine: 0-1.5 mm range allowed.
Can measure the height of unknown plates using the Operetta with the 20x objective.

Edge effects
Prefer not to use the outer rows and outer columns of any given plate.
This because of "edge effects" (thermal, evaporation, different near the plate edge).
Fill those wells with only buffer, for example, and do not use for imaging.

Saved parameters
During an experiment, you define and save the following objects:

• Experiment  (light, stacks, channels, etc)
• Assay layout  (cells, treatments, rows/column names)
• Measurement  (image data)
• Analysis sequence  (processing steps, calculations, values of interest)
• Evaluation  (grouping, graphs/charts, heatmaps, well-display, statistics, data points)