Seitenhistorie

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• "start" in symphotime (1)
• "start" in zeiss software (2)
• "stop" in symphotime
• save image, name it, copy data to word file: Picture of cells, Picture of histogram
  
  
• Do that for each acquisition separately
• info: for a 2-component fit, >1000 photons per pixel are needed
• ROI data is displayed in histogram, but warning: has to be told to use that ROI for the fit
  
  
• Select experiment
• Analyze
• Calculate fast FLIM
• Fitting model
• Import the measured IRF (from the set-up step)
• Initial fit (change # of parameters used, if necessary)
• Evaluate amps + their error range
• Evaluate mean/average lifetime
• "shift" indicates delay between IRF/population of the early lifetimes that were fit