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Service contact (administrators only): "EVO Portal", Perkin Elmer website. |
Incubation: Heating and CO2 controller available. No humidity control (use closed samples if necessary).
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- Manufacturer comments on plate usage: https://www.perkinelmer.com/uk/lab-products-and-services/application-support-knowledgebase/microplates/microplates-knowledge-base.html
- Machine takes "all" available cover-slip bottomed plates.
- But, do not use Techno Plastic Products (yellow stripe); which have plate-vents and cannot be supported by the holder.
- may have a glass or plastic "coverslip" bottom
- for good cell-attachment, can coat the bottom with poly-L-lysine
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Important value to enter into machine. 0-1.5 mm range allowed. Can measure the height of unknown plates using the Operetta with the 20x objective. |
- When correctly entered, objectives will never touch the plate bottom (if still; plate will be pushed upwards).
- Edge effects: Prefer not to use the outer rows and outer columns of any given plate. This because of "edge effects" (thermal, evaporation, different near the plate edge).
- Edge effects: Fill those wells with only buffer, for example.
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