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Tipp

Skirt height
Difference between plate-edge height, and imaging surface height
Important value to enter into machine: 0-1.5 mm range allowed.
Can measure the height of unknown plates using the Operetta with the 20x objective.

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Tipp

Edge effects

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Prefer not to use the outer rows and outer columns of any given plate.
This because of "edge effects" (thermal, evaporation, different near the plate edge).

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Fill those wells with only buffer, for example, and do not use for imaging.

Usage

Around the sample area:

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