Seitenhistorie

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Incubation: Heating available (37-42 °C; system temperature of about 25 °C without heating activated). CO₂ controller planned-to-be-availableavailable (1-10 %). No humidity control: Use closed samples in excessive imaging buffer, if necessary.

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Light/optics: 4x LED light source (can activate 1 source simultaneously), 4x 5x emission filter (UV / green /yellow /  red / far-red), Spinning disc optics. 1x top-down-LED (740 nm, for morphology, using transparent-top samples).

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• 430-500 nm  (blue)
• 500-550 nm  (green)
• 515-580 nm  (yellow)
• 570-650 nm  (red)
• 665-760 nm  (far-red)

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• When done: open eject the lid sample (settings - Operetta CLS - openeject) and remove plate/slide holder. Afterwards "eject" once more to close the system again.

Exporting data

• In the "settings" menu up top, data management can be used to export data
• Export your data as an "archive" for later re-analysis using Harmony, eg. on the CAi image-analysis PC
• Export your data as "measurement + associated data" to save results as TIFF files, alongside already-performed measurement outcomes, and re-analysis eg. using CellProfiler
• Connect to the CAi shared network drive to store the data, or bring your own data-carrier (USB).
• After the session, delete your data from the Operetta computer.

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