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Method | Instrumentation | Applications |
---|---|---|
Size exclusion & affinity chromatography | AKTA Pure | Protein isolation nanodisc assembly |
Density gradient station (automated) | Biocomp Suitable rotor: SW40Ti, SW32 Ti | Fractionation of macromolecules based on size/density Isolation of large complexes, e.g. ribosomes Fractionation of organelles |
Quartz crystal microbalance | coming soon | |
Nanodisc reconstitution | MSP-based nanodiscs of various sizes | reconstitution of membrane proteins for biochemical and biophysical analysis |
Polymer-based protein isolation | SMA, DIBMA polymers ("native" nanodiscs) | Isolation of membrane proteins with their native lipid environment |
Cell-free protein expression | E. coli S30 extract; PURExpress | |
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