| Method | Instrumentation | Applications |
|---|---|---|
| Size exclusion & affinity chromatography | AKTA Pure FPLC | Protein isolation; Nanodisc assembly; isolation of macromolecular complexes |
Density gradient station (automated) | Biocomp Gradient Station Suitable rotor: SW40Ti, SW32 Ti | Automated preparation of sucrose/glycerol/... gradients for ultracentifugation Fractionation of macromolecules based on size/density Isolation of macromolecular complexes, e.g. ribosomes Fractionation of organelles & bacterial membranes |
| Quartz crystal microbalance | coming soon | |
| Nanodisc reconstitution | MSP-based nanodiscs of various sizes (4-16 nm diameter) | Reconstitution of membrane proteins for biochemical and biophysical analysis |
| Polymer-based protein isolation | SMA, DIBMA polymers ("native" nanodiscs) | Isolation of membrane proteins with their native lipid environment |
| Cell-free protein expression | E. coli S30 extract; PURExpress | |
| Protein visualization & quantification | Amersham Imager AI600RGB | In-gel fluorescence detection (blue, green, red) Western blotting (combined with photo-imaging) In-gel densiometry analysis |
| Synthetic biomolecular coupling | SpyTag / SpyCatcher system Monovalent streptavidin Monomeric streptavidin | Specific protein:protein coupling Protein detection & labelling |
| Liposome preparation | IKA Rotary evaporator (incl. thermostated bath) Micro-extruder kit | Preparation of multi-component lipid mixtures, incl. synthetic components Formation of unilamellar vesicles of defined size (down to 50 nm) |
| Supported lipid bilayers (SLB) | Glass slides, diverse silane cushions | Lipid bilayers suitable for microscopy applications Silane cushion to ensure membrane protein mobility within the bilayer |
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