Seitenhistorie

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Example 2: multi-tile experiment shows dark shadows at the borders of images after stitching (left image without illumination correction; right image shows stitching after illumination correction; images are taken from http://nic.ucsf.edu/blog/2014/01/shading-correction-of-fluorescence-images/).

without correction:      with correction:

For best image quality and especially for intensity-based image analysis (e.g. thresholding), it is therefore importing to perform an illumination correction. In general, there are two ways to accomplish this:

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