Seitenhistorie

Safety

• Genetic (ask for "wrapper of Form Z" for each new project - to describe GMOs
• Chemical (corrosion, toxic)
• Laser (mirrors, opening boxes, etc)

Booking

• Log-book writing (can refer to formblatt Z excerpt/number)
• Booking policies (website: 3 h slots, 4-wk in advance max)

Incubator

• we keep it at 21.5C, all the time (do not leave it open!)
• other cooling: right-side of the table = 17C, 24/7 for the objective turret
• leave coolings on

Switch-on

• Pause between buttons, do you need the fluorescent lamp?

Log-in

• TCS user - machine - DMI8 stage
• Generally non-active (but optionally on):
• Resonant scanner (greatly increase scan speed), STED (high resolution), AFC (adaptive focus control - good for keeping time course in focus)
• Initialize table (for multiple locations imaging; raise microscope arm first, objectives go down automatically)

Software - lasers

• Laser config: STED vs. non-STED
• Which lasers do we have? What do they do? WLL (pulsed, pick wavelength, gating), Argon (more powerful, fan cooling)
• How to switch them off (especially Argon)
• The fluorescent lamp needs at least 30 minutes between off-on

Touch panel

• Objective switching, fluorecent light / cubes on-off, pick, transmitted light
• Immersion of objectives: which one to choose?
• Save positions using touch pad: working/safe positions. Careful not to crash automatically...

Table inlet

• Is for fine z-adjustments, eg. active when xz-scanning
• Do not lean on, or push down hard on!

Sample placement/preparation

• Table sample holder: move holders to accomodate sample - leave open to allow raising sample
• Objective selection + apply immersion oil
• Focus finding: see droplet become wider, use fluorescence/DIC

Software - beam path