Seitenhistorie
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Example 2: multi-tile experiment shows dark shadows at the borders of images after stitching (left image without illumination correction; right image shows stitching after illumination correction; images are taken from http://nic.ucsf.edu/blog/2014/01/shading-correction-of-fluorescence-images/).
without correction: 
with correction: 
For best image quality and especially for intensity-based image analysis (e.g. thresholding), it is therefore importing to perform an illumination correction. In general, there are two ways to accomplish this:
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- Zeiss ZEN blue/black
In ZEN the "Shading correction" can be found at the "Processing" tab and is used during image acquisition to be used with a captured reference image. For further instructions, please follow this existing guide from the University of Queensland, where the image was taken from.
- Leica Las X
At the SP8 the "Shading correction" is used during image acquisition to be used with a captured reference image. For further instructions, please follow this existing guide from the University of Queensland, where the image was taken from.
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