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  • Plate layout (right-most). Select wells to measure, click "SELECT". Select FOV, number, position.
  • Select well-to-test.
  • Clean plate-bottom with wipe (KimTech wipe or similar) and 70% Ethanol before use.
  • Load plate with or without lid (place within protrusions).
  • or: Load slides in holder.
  • or: Load slide holder into Operetta.

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  • Evaluate that the correct assay is loaded.
  • Name the plate and add keywords.
  • → Start! And observe live-preview images during acquisition (when enabled)

  • Select the whole plate, right-click, organize images in "realistic" (corresponding to the plate) or "packed" (best for display) layouts


Tipp

Rule of thumb for estimated dataset size
The Operetta CLS is only displaying the total size of a dataset if not enough disk space is available. If you want to know the estimated dataset size before you start an experiment, use the following rule of thumb:

Number of wells x fields x channels x z-planes x timepoints x 8 MB = approximate dataset size in MB


Tipp

Flat-field correction
The software can do flat-field correction based on acquired images, provided that foreground/background and areas without cells are present.
This also works in wells filled with tissue, or more-than-confluent cell cultures.

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