Seitenhistorie

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• Booking policies (show website: 3 h slots, 4-wk in advance max)
• Don't book and then just before, remove a massive session
• We use the booking system to generate bills

Incubator

• we We keep it at 21.5°C, all the time (do not leave it open!)
• other Other cooling: right-side of the table is at 17°C, all the time, for the objective turret
• leave Leave coolings on!

Switch-on

• Buttons in numbered sequence
• Pause between buttons, do you need the fluorescent lamp?

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• Use gentle imaging conditions, and the control-panel Z-controls, define top and bottom of the stack
• The knobs on the microscope control the height of the whole table, the knob on the control panel controls the smaller Z-inlet
• Change step-size, best is system optimized, but can reduce them a bit

Time series/focus control

• Define total experiment time, and intervals between acquisitions
• The series will use the currently active acquisition settings (speed, format, etc.)
• Now the Automated Focus Control (AFC) becomes relevant, which uses IR laser reflection to hold-current-position over time
• "show Show autofocus channel" can show what's going on there

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