Method | Instrumentation | Applications |
---|---|---|
Size exclusion & affinity chromatography | AKTA Pure FPLC | (Membrane) protein isolation; isolation of macromolecular complexes |
Isothermal titration calorimetry (ITC) | Microcal/Malvern ITC 200 | Thermodynamic analysis of protein:protein and protein:ligand interactions (affinity, stoichiometry, free energy, enthalpy, entropy) |
Membrane mimetics | Saposin/Salipro, Peptidisc | Reconstitution of membrane proteins in nanometer-sized particles Suitable for biochemical, biophysical and structural anaysis |