Methods InstrumentationApplications
BioImage Data AnalysisSoftware: Matlab, Octave, FIJISegmentation and Quantification of Objects and Dynamics from microscopic images
Biophysical Modeling Software: Matlab, ComSolTheoretical Model Design and Testing
Single Molecule TrackingOlympus Epifluorescence Microscope IX73 with Laser Illumination Identification of Diffusion, Binding, Transport of individual molecules (30 nm resolution)
Microinterferometry (RICM)Olympus Epifluorescence Microscope IX73 with crossed polarizersReconstruction of thin layer topographies; quantification of biomembrane dynamics (5 nm resolution)
Live Cell Microscopy (transfection, time-lapse microscopy)Olympus Epifluorescence Microscope IX83 with Autofocus Observation of Cellular dynamics (up to 24h)
Microinjection Eppendorf FemtojetInjection of proteins, nanoparticles,... in cells
Soft Lithography / MicropatterningAlvéole (https://www.alveolelab.com/applications/high-content-screening/)Patterning of any desired protein pattern or gradient on glass substrate
Magnetic Nanoparticles (semisynthetic; synthesis and characterization)E.coli Expression, Purification (Äkta), Autotitration System (Mettler Toledo)Magnetogenetic manipulation of Nanoparticle labeled molecules
Quantum Dots (characterization and molecular labeling)  little instrumentation needed;  available in any chemistry labSmall Fluorescent sensors (~ 5 nm) with adjustable spectroscopic properties
Giant Unilamellar Vesicles (GUV)Electroswelling Chamber Biomimetic system to characterize molecules in a well-defined environment
Supported Lipid Bilayers (SLB)Langmuir TroughBiomimetic system to characterize molecules in a well-defined environment
Fusogenic Liposomeslittle instrumentation needed;  available in any chemistry labLiposome fusion with cell membrane to transfer lipids, proteins,...
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