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Introduction protocol
Safety
- Genetic (ask for "wrapper of Form Z" for each new project - to describe GMOs
- Chemical (corrosion, toxic)
- Laser (mirrors, opening boxes, etc)
Booking
- Log-book writing (can refer to formblatt Z excerpt/number)
- Booking policies (website: 3 h slots, 4-wk in advance max)
Sample
What is your sample? What are you interested in visualising?
Incubator
- we keep it at 21.5C, all the time (do not leave it open!)
- other cooling: right-side of the table = 17C, 24/7 for the objective turret
- leave coolings on
Switch-on
- Pause between buttons, do you need the fluorescent lamp?
Log-in
- TCS user - machine - DMI8 stage
- Generally non-active (but optionally on):
- Resonant scanner (greatly increase scan speed), STED (high resolution), AFC (adaptive focus control - good for keeping time course in focus)
- Initialize table (for multiple locations imaging; raise microscope arm first, objectives go down automatically)
Working distance
The 100x objective, NA=1.40, works very close to the coverslip
Software - lasers
- Laser config: STED vs. non-STED
- Which lasers do we have? What do they do? WLL (pulsed, pick wavelength, gating), Argon (more powerful, fan cooling)
- How to switch them off (especially Argon)
- The fluorescent lamp needs at least 30 minutes between off-on
Touch panel
- Objective switching, fluorecent light / cubes on-off, pick, transmitted light
- Immersion of objectives: which one to choose?
- Save positions using touch pad: working/safe positions. Careful not to crash automatically...
Rotary knobs
Rotary knobs on the touch pad and microscope - rotate away from you = move objective closer to sample
Table inlet
- Is for fine z-adjustments, eg. active when xz-scanning
- Do not lean on, or push down hard on!
Sample placement/preparation
- Table sample holder: move holders to accomodate sample - leave open to allow raising sample
- Objective selection + apply immersion oil
- Focus finding: see droplet become wider, use fluorescence/DIC
Software - beam path
Überblick
Inhalte
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